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( Mark One ) X UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington , D.C. 20549 ANNUAL REPORT PURSUANT TO SECTION 13 OR 15 ( d ) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31 , 2020 or TRANSITION REPORT PURSUANT TO SECTION 13 OR 15 ( d ) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number 001-34899 Delaware ( State or other jurisdiction of incorporation or organization ) Form 10 - K 3957 Point Eden Way Hayward , CA ( Address of principal executive offices ) Title of Each Class Common Stock , par value $ 0.001 per share Large accelerated filer Non - accelerated filer Pulse Biosciences , Inc. ( Exact name of registrant as specified in its charter ) Registrant's telephone number , including area code : ( 510 ) 906-4600 Securities registered pursuant to Section 12 ( b ) of the Act : X Trading Symbol ( s ) PLSE 46-5696597 ( I.R.S. Employer Identification No. ) Securities registered pursuant to Section 12 ( g ) of the Act : None Indicate by check mark if the registrant is a well - known seasoned issuer , as defined in Rule 405 of the Securities Act . Yes " No x Indicate by check mark if the registrant is not required to file reports pursuant to Section 13 or 15 ( d ) of the Act . Yes No x Indicate by check mark whether the registrant ( 1 ) has filed all reports required to be filed by Section 13 or 15 ( d ) of the Securities Exchange Act of 1934 during the preceding 12 months ( or for such shorter period that the registrant required file such reports ) , and ( 2 ) has been subject to such filing requirements for the past 90 Yes x No " Indicate by check mark whether the registrant has submitted electronically every Interactive Data File required to be submitted pursuant to Rule 405 of Regulation S - T ( §232.405 of this chapter ) during the preceding 12 months ( or for such shorter period that the registrant was required to submit such files ) . Yes x No 94545 ( Zip Code ) Indicate by check mark whether the registrant is a large accelerated filer , an accelerated filer , a non - accelerated filer , smaller reporting company , or an emerging growth company . See the definitions of “ large accelerated filer , ” “ accelerated filer , ” “ smaller reporting company , ” and “ emerging growth company ” in Rule 12b - 2 of the Exchange Act : Name of Each Exchange on Which Registered The Nasdaq Stock Market LLC Number of shares outstanding of the registrant's common stock as of February 28 , 2021 : 26,086,931 DOCUMENTS INCORPORATED BY REFERENCE : If an emerging growth company , indicate by check mark if the Registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 13 ( a ) of the Exchange Act . Accelerated filer Smaller reporting company Emerging growth company Indicate by check mark whether the registrant has filed a report on and attestation to its management's assessment of the effectiveness of its internal control over financial reporting under Section 404 ( b ) of the Sarbanes - Oxley Act ( 15 U.S.C. 7262 ( b ) ) by the registered public accounting firm that prepared or issued its audit report . Indicate by check mark whether the registrant is a shell company ( as defined in Rule 12b - 2 of the Exchange Act ) . Yes " No x Aggregate market value of registrant's common stock held by non - affiliates of the registrant on June 30 , 2020 , the last business day of the registrant's most recently completed second fiscal quarter , based upon the closing price of the registrant's common stock on such date as reported by Nasdaq Capital Market , was approximately $ 135,220,301 . Shares of voting stock held by each officer and director have been excluded in that such persons may be deemed to be affiliates . This assumption regarding affiliate status is not necessarily a conclusive determination for other purposes . X X Portions of the registrant's definitive Proxy Statement relating to its 2021 Annual Meeting of Stockholders are incorporated by reference into Part III of this Form 10 - K where indicated . The Proxy Statement will be filed with the U.S. Securities and Exchange Commission within 120 days after December 31 , 2020 .