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Table of Contents ( Mark One ) UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington , D.C. 20549 ANNUAL REPORT PURSUANT TO SECTION 13 OR 15 ( d ) OF THE SECURITIES EXCHANGE ACT OF 1934 FORM 10 - K TRANSITION REPORT PURSUANT TO SECTION 13 OR 15 ( d ) OF THE SECURITIES EXCHANGE ACT OF 1934 Title of each class Common Stock For the fiscal year ended September 30 , 2021 OR Delaware ( State or other jurisdiction of incorporation or organization ) Large accelerated filer > Non - accelerated filer For the transition period from Commission File Number : 001-38720 TWIST to Twist Bioscience Corporation ( Exact Name of Registrant as Specified in its Charter ) 681 Gateway Blvd , South San Francisco , CA 94080 ( Address of principal executive offices and zip code ) ( 800 ) 719-0671 ( Registrant's telephone number , including area code ) Trading Symbol ( s ) TWST 46-205888 ( I.R.S. Employer Identification No. ) Name of each exchange on which registered The Nasdaq Global Select Market Indicate by check mark if the Registrant is a well - known seasoned issuer , as defined in Rule 405 of the Securities Act . YES NO □ Indicate by check mark if the Registrant is not required to file reports pursuant to Section 13 or 15 ( d ) of the Act . YES □ NO ' Indicate by check mark whether the Registrant : ( 1 ) has filed all reports required to be filed by Section 13 or 15 ( d ) of the Securities Exchange Act of 1934 during the preceding 12 months ( or for such shorter period that the Registrant was required to file such reports ) , and ( 2 ) has been subject to such filing requirements for the past 90 days . YES > NO ☐ Indicate by check mark whether the Registrant has submitted electronically every Interactive Data File required to be submitted pursuant to Rule 405 of Regulation S - T ( §232.405 of this chapter ) during the preceding 12 months ( or for such shorter period that the Registrant was required to submit such files ) . YES > NO ☐ Indicate by check mark whether the Registrant is a large accelerated filer , an accelerated filer , a non - accelerated filer , a smaller reporting company , or an emerging growth company . See the definitions of " large accelerated filer , " " accelerated filer , " " smaller reporting company " and " emerging growth company " in Rule 12b - 2 of the Exchange Act . ( Check one ) : Accelerated filer Small reporting company Emerging growth company 000 If an emerging growth company , indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 13 ( a ) of the Exchange Act . Indicate by check mark whether the registrant has filed a report on and attestation to its management's assessment of the effectiveness of its internal control over financial reporting under Section 404 ( b ) of the Sarbanes - Oxley Act ( 15 U.S.C. 7262 ( b ) ) by the registered public accounting firm that prepared or issued its audit report . NO É Indicate by check mark whether the Registrant is a shell company ( as defined in Rule 12b - 2 of the Exchange Act ) . YES □ As of March 31 , 2021 , the last business day of the registrant's most recently completed second fiscal quarter , the aggregate market value of shares of common stock held by non - affiliates of the registrant was approximately $ 4.885 billion based upon the closing sale price on the Nasdaq Global Select Market reported for such date . Shares of Common Stock held by each officer and director and by each person who owns 10 % or more of the outstanding Common Stock have been excluded in that such persons may be deemed to be affiliates . This determination of affiliate status is not necessarily a conclusive determination for other purposes . The number of shares of the Registrant's common stock outstanding as of November 18 , 2021 , was 49,632,483 . DOCUMENTS INCORPORATED BY REFERENCE Portions of the Registrant's definitive proxy statement to be filed in connection with its 2022 Annual Meeting of Stockholders are incorporated by reference into Part III of this Form 10 - K .